This is the first edition of the astrobotany handbook.  The handbook serves as a manual for researchers and research protocol for spaceflight plant biology.  The techniques and protocols discussed range from simple procedures to the more technical and specialized.  Let’s grow plants in space.


  1. astrobotany experimental design
  2. TOAST (Astrobotany Genetic Database)
  3. ethanol seed sterilization
  4. making space media (agar)
  5. vapor phase seed sterilization
  6. RootTrace Software
  7. creation of a rescue line
  8. microarray analysis
  9. RNAseq analysis
  10. standard gravitropic assay
  11. standard barrier assay
  12. astrobotany literature