Molecular Biology Resources

This page contains resources for molecular biology and/or other wet lab work. It also contains some supplemental resources for classroom development.

  
Create polls that you can do during classhttps://pollev.com/
A great place to store protocolshttps://www.protocols.io
  
Mistakes to avoid when presenting datahttps://www.biostat.wisc.edu/~kbroman/topten_worstgraphs/
Planning to save meta-data 
Make a cloud datastore where you can share your results with educatorshttp://www.iplantcollaborative.org/
New Tutorial on RNAseq analysis pipeline in CyVersehttps://wiki.cyverse.org/wiki/display/TUT/RNA-seq+Tutorial-+HISAT2%2C+StringTie+and+Ballgown
 Data carpentry folder structure
Intro to BRIC19 RNAseq experimenthttp://www.rnaseqforthenextgeneration.org/profiles/barker.html
DNA subway Green Line (Preprocessing to counts )https://dnasubway.cyverse.org/
Provide counts tableCounts from BRIC19 on MacMini desktop (down load link from CyVerse)
Volcano plots require Log FC & -Log10 P-valuehttp://moderndata.plot.ly/interactive-volcano-plots-r-plotly/
  
Transcriptome 
RNAseq normaliation tool DESeq, EdgeR, Voomhttps://gallery.shinyapps.io/DEApp/
RNAseq heat map and PCA toolhttps://kcvi.shinyapps.io/START/
Instructions for build your own heatmapshttp://www2.warwick.ac.uk/fac/sci/moac/people/students/peter_cock/r/heatmap/
  
Deposit figures in slide showFind method to save lists of genes and meta data for report
  
Copmare and contrast datasets 
Shiny Venn diagramhttp://jvenn.toulouse.inra.fr/app/example.html
  
Multi-gene 
Go analysis toolhttp://plantregmap.cbi.pku.edu.cn/go.php
Alternative Go Analysis toolhttp://bioinfo.cau.edu.cn/agriGO/analysis.php
Gene network analysis toolhttp://knetminer.rothamsted.ac.uk/Arabidopsis_thaliana/
Compare your lists to the transcriptionfactor data baseshttp://atrm.cbi.pku.edu.cn/
Search for co-expression paterns using BARhttp://bar.utoronto.ca/ExpressionAngler/
Plot the diurnal expression patterns in different light environmentshttp://diurnal.mocklerlab.org/diurnal_data_finders/new
SUBA tool box (co-expression, subcellular location enrichment)http://suba.live/toolbox-app.html
  
Single gene 
Use this tool to investigate the most interesting genehttps://apps.araport.org/thalemine/begin.do
Use this to make a graphical report on interesting gene expressoin patternshttps://bar.utoronto.ca/eplant/
Observe phylogenetic tree of genehttp://plants.ensembl.org/Arabidopsis_thaliana/Gene/Compara_Tree?g=AT4G39670;r=4:18409846-18411069;t=AT4G39670.1
Explore a single genehttp://www.megabionet.org/atpid/webfile/query.php
Check if your gene is found in the plant membrane protein databasehttp://aramemnon.uni-koeln.de/request.ep
  
FASTA file format 
Check if ther protein contains any transmembrane domainshttp://www.cbs.dtu.dk/services/TMHMM-2.0/
  
  
Design experiment to test hypothesis 
Plant image anlysis softwarehttp://plant-image-analysis.org/
FlashLapse as a tool for acquiring consitant image qualityhttps://github.com/flashlapsedev/Flashlapse_Build
Protocols Astrobotany.com and Trello 
  
Tools for quick graph production 
Shiny Box Plothttp://shiny.chemgrid.org/boxplotr/
Shiny GGPlot graph productionhttps://site.shinyserver.dck.gmw.rug.nl/ggplotgui/
Shiny for RootNavhttps://rmtrane.shinyapps.io/RootNav/
Shiny for RootTacehttps://rmtrane.shinyapps.io/RootTrace/
Online statiscal tool with guidehttp://vassarstats.net/
Alternative Shiny Onlines statiscal toolhttp://www.intro-stats.com/
Online histograms and related statshttps://www.wessa.net/rwasp_histogram.wasp
Online interactive figure creationhttps://plot.ly/~PlotBot/51/control-vs-experimental/
  
Eric’s root analysis pipeline 
J-filament software for measuring roots / wavy lineshttp://athena.physics.lehigh.edu/jfilament/download.chy#current
Custom R-codes for analyzing roots / wavy lineshttps://github.com/eschultzphd/RootMeasurement/blob/master/RootMeasure.R
  
New image analysis pipeline 
Root NAV & Other plant image analysis softwarehttp://www.plant-image-analysis.org/api
The language of RSA = RSMLhttp://rootsystemml.github.io/tools/
This software can make example roots to train other softwarehttps://www.biorxiv.org/content/early/2016/12/22/074922
Deposit of ground truth synethetic imageshttp://www.plant-image-analysis.org/dataset/modelled-root-images
Archi-DART: New R-Shiny app for root modeling / making graphshttps://archidart.github.io/
PRIMAL machine learninghttps://github.com/plantmodelling/primal
Root image analysis pipeline evaluationhttps://github.com/guillaumelobet/Root-Image-Analysis-Pipeline-Evaluation/tree/v0.1